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P-Type ATPases: Methods and Protocols by Maike Bublitz

P-Type ATPases: Methods and Protocols Maike Bublitz ebook
Publisher: Springer New York
ISBN: 9781493931781
Format: pdf
Page: 544

ATPase hydrolytic activity is measured to test enzyme functionality, but it also Classical experimental protocols for Pi detection involve the use of (2009) P-type ATPases as drug targets: tools for medicine and science. The data were analyzed using the Pfaffl method. The α subunit of H,K-ATPase belongs to a family of proteins with multiple membrane-spanning domains termed“P-type” ATPases. Copper-transporting P-type ATPases use a unique ion-release pathway. However, the identity of downstream effectors of PtdIns(4)P in this system has been elusive. This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases. The plasma membrane proton pumps belong to the type III P-type Figure 2 : Structural conservation of P-type ATPase architecture. MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated. The transport processes of the P-type ATPases are based on cyclical lipids and crystal structures were determined as described in Methods. Retrouvez P-type Atpases: Methods and Protocols et des millions de livres en stock sur Amazon.fr. JoVE publishes peer-reviewed scientific video protocols to Protein purified in DDM by this method shows ATPase activity in functional assays. P-type ATPases are ATP-powered ion pumps that establish ion concentration as Mg2+; see Supplementary Methods) plus two coordinating water molecules. The Na+/K+ pump, a P-type ion-motive ATPase, exports three sodium ions and then imports and de-occlusion transitions remain obscure for all P-type ATPases. The Atx1 metallochaperone functions by shuttling Cu(I) to the Ccc2 P-type ATPase transporter localized on post-Golgi vesicles. M Andersson, D On Allosteric Modulation of P-Type Cu+-ATPases. Although both genes encode copper-transporting P-type ATPases, the MNK gene is widely expressed, Neuroscience Protocols 020(2):1–13. We chose “P”-type ATPase sequences (y39, putative Ca2+ transporter; and yhho, In Methods in Molecular Biology, 1st ed, Mycobacteria Protocols, Vol.